ردیابی ژن بیوسنتز سیانید هیدروژن در سودوموناس‎های فلورسنت بازدارنده رشد R. solani عامل پوسیدگی ریشه چغندرقند

نوع مقاله : کامل علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی دانشگاه آزاد اسلامی، واحد سبزوار

2 استادیار گروه زیست شناسی دانشگاه حکیم سبزواری

3 استادیار گروه دامپزشکی دانشگاه آزاد اسلامی، واحد سبزوار

چکیده

برای ردیابی ژن سیانید هیدروژن و تعیین قابلیت سودوموناس­های فلورسنت در تولید سیانید هیدروژن و بازدارندگی از رشد بیمارگر Rhizoctonia solani عامل پوسیدگی ریشه و طوقه چغندرقند از ریزوسفر مزارع چغندرقند سبزوار نمونه‎برداری شد. پس از جمع‎آوری نمونه­های خاک و خالص­سازی، 31 سویه سودوموناس جداسازی شده و در محیط اختصاصی سودوموناس آگارF، سودوموناس­های فلورسنت براساس آزمون­های میکروبی تفکیک و شناسایی شدند. نتایج PCR برای ردیابی ژن بیوسنتز سیانید هیدروژن نشان داد که سه سویه حاوی ژن بیوسنتز سیانید هیدروژن بودند. در بررسی کیفی تولید سیانید هیدروژن در محیط کشت میکروبی نیز مشخص شد که این سویه­ها توانایی تولید سیانید هیدروژن را به مقادیر مختلف دارند. جهت بررسی میزان بازدارندگی جدایه­های سودوموناس فلورسنت از رشد قارچ R. solani، درصد کاهش رشد قارچ در حضور باکتری محاسبه گردید. از میان سه سویه سودوموناس فلوروسنت، نمونه C7 بیشترین میزان بازدارندگی از رشد قارچ و بیشترین مقدار تولید سیانید هیدروژن را داشت که به‎عنوان کاندیدای مناسب بیوکنترل معرفی می شود.

کلیدواژه‌ها

عنوان مقاله [English]

Detection of hydrogen cyanide biosynthetic gene in Pseudomonas fluorescent as a control agent of Rhizoctonia solani growth

نویسندگان [English]

  • M. Konjedi 1
  • J. Vatandoost 2
  • A Janatabadi 3
1 MSc Student, Dept. of Agricultural Biotechnology, Islamic Azad University, Iran
2 Assistant professor, Dept. of Biology, Hakim Sabzevari University, Iran.
3 Assistant professor, Dept. of Agriculture, Islamic Azad University, Iran.
چکیده [English]

In order to detect hydrogen cyanide gene and to determine Pseudomonas fluorescent efficiency in producing hydrogen cyanide for controlling the growth of Rhizoctonia solanipathogen (the causal agent of sugar beet root and crown rot), samples were collected from sugar beet rhizosphere in Sabzevar fields. After soil sampling and purification, 31 Pseudomonas Spp. isolates were separated on specific Pseudomonas agar F medium, and Pseudomonas fluorescent strains were identified and isolated based on microbial tests. PCR results showed that three strains contained cyanide hydrogen biosynthetic gene. The qualitative evaluation of hydrogen cyanide production in microbial medium showed that all the strains were capable of producing different amounts of hydrogen cyanide. To determine the growth inhibition rate of Rhizoctonia solani by Pseudomonas fluorescent, the percentage of fungus growth in the presence of bacteria was calculated. Among the three Pseudomonas fluorescent strains, C7 showed the greatest inhibition rate and hydrogen cyanide production which can be recommended as a suitable candidate for the pathogen biocontrol.

کلیدواژه‌ها [English]

  • biocontrol
  • Pseudomonas fluorescence
  • Rhizoctonia root and crown rot
  • sugar beet

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