The effect of sugar beet seed advancement on response to salt stress In vitro conditions

Yavari, N.; Mesbah, M.; Ghaffari Jahromi, A.R.

The effect of sugar beet seed advancement on response to salt stress In vitro conditions

Document Type : Scientific - Research

Authors

¹ Researcher of Sugar Beet Seed Institute, Karaj, Iran.

² Computer and Statistics Dept. SPII. Karaj. Iran

Abstract

Sensivity of seed germination stage to saline soil is the main obstacle to proper field establishment of sugar beet crop. In previous studies different tissues of sugar beet showed high tolerance to salt stress by of increasing sodium chloride levels in in vitro condition. An experiment was by to study the response of sugar beet zygotic embryos to salt stress in in vitro conditions. Sterile perfect seeds and naked embryos from salt sensitive (9597-67) and salt tolerant (7233) lines were cultured in PGoB medium and four levels of salinity: 0, 100, 150 and 250 mM NaCl, with three replications and 10 explants/Petri dish. Dishes were kept at 25º ± 2º C and 2000 Lx for a period of four weeks. A factorial design was used in statistical analysis. Mean values were compared using DMRT and F test. Naked embryos of both lines sprouted in both stress levels whereas their perfect seeds did not. There were significant differences between the plant material, salt levels and their interaction. Furthermore seed advancement experiment with an eight hours Thiram, (0.2%) treatment and gradual decrease of seed moisture content at 25º C was performed. This treatment included 25 seeds /dish of three sugar beet lines and the same salt levels in water agar medium with 4 replications. Treated seeds of all three lines germinated in stress conditions. Results showed that lines, stress levels and their interaction were significantly different. The line 7233 was the most tolerant genotype followed by 9597-67 and 261. The benefit of 'seed Advancement ' treatment for tolerance to salt stress conditions was demonstrated.

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