Determining the appropriate time to evaluate the resistance of sugar beet roots to rhizoctonia rot in microplot based on the growing degree days

Document Type : Scientific - Research

Authors

1 Assistant professor of Sugar Beet Research Department, Hamedan Agricultural and Natural Resources Research and Education Center (AREEO), Hamedan, Iran.

2 Assistant Professor of Sugar Beet Research Department, Hamedan Agricultural and Natural Resources Research and Education Center, AREEO, Hamedan, Iran.

3 Assistant professor of Sugar Beet Seed Institute (SBSI), Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.

Abstract

Evaluating the resistance of lines under artificial inoculation in micro-plot condition is one of the most effective methods to obtain disease-resistant lines. In order to determine the appropriate time to evaluate the resistance of sugar beet lines to rhizoctonia rot, an experiment was carried out in Hamedan in 2022 under artificial inoculation in micro-plot. The experimental treatments consisted of six different times of harvest and root evaluation including 12, 18, 22, 24, 26 and 29 days after inoculation with two replications for two susceptible and resistant genotypes. Growing degree days in all treatments was recorded from inoculation time until harvest, and finally the growth degree day was achieved for each treatment in this period. Roots were evaluated on a scale of 1-9 at the harvest. Root evaluation period was calculated by curve fitting between the growing degree days received as variable x and the percentage of rotted roots in susceptible genotype as variable y to determine the start of the evaluation period and using curve fitting between growing degree days received as variable x and the disease index of resistant genotype as variable y to determine the end of the evaluation period. Results of the experiment showed that the appropriate time to evaluate the resistance of sugar beet roots to rhizoctoneia is from 483 growing degree days to 518 growing degree days after inoculation.

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