Evaluation the genetic diversity of Beta vulgaris subsp. maritima samples using nuclear and cytoplasmic molecular markers

Document Type : Scientific - Research


1 Educated of Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Associate Professor of Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University of Hamedan, Iran.

3 Assistant Professor of Sugar Beet Seed Institute(SBSI), Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.

4 . Associate Professor of Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University of Hamedan, Iran.


Beta vulgaris ssp. Maritima plays a significant role in the breeding programs of sugar beet and evaluation of its genetic diversity is of importance. In this study, thirteen samples of Maritima beet including 10 Iranian and three European accessions were evaluated. Two types of molecular markers including ISSR (inter-simple sequence repeat) and minisatellite were used to characterize the nuclear and mitochondrial genomes. A total of 176 loci were identified based on 13 ISSR primers, of which 147 (84.27%) loci showed polymorphisms. Results showed that among the samples, the value of Polymorphism Information Content (PIC) was 32%. Primers 8YT(AC) and 8YC (GA) had the highest PIC value of 0.38. Primer 8RG(CT) with the highest marker index (MI) could be used for a more detailed analysis. Based on the results of UPGMA and ANOVA analysis, all samples were clustered into two main groups. Principle component analysis (PCoA) with dendrogram of genetic diversity showed that Iranian and European samples are different from each other. In order to study the cytoplasm diversity, four minisatellite markers were used, and the samples were divided into four mitochondrial groups based on the variety of primers. According to the sequencing results, some Iranian samples were heteroplasmic.


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