A study on the effect of Ca ion concentration on NaCl toxicity in sugar beet cotyledonary tissue in vitro culture

Document Type : Scientific and Research

Authors

1 Researcher of Sugar Beet Seed Institute, Karaj, Iran.

2 Researcher of Fars Agricultural and Natural Resources Research Center, Shiraz, Iran.

Abstract

Sugar beet cotyedonary tissue from 21-days-old  invitro seedlings which has shown a good regeneration ability is used in a study on the effect of Ca ion concentration on NaCl toxicity. Explants were cultured on PGO medium containing 0.25 mg/l BA, 0.05 mg/l Carbenicillin, .% sucrose and 0.9% Agar.  NaCl and CaCl2 were supplemented in seven (0-100-150-200-250-300-350 mM) and four (1-3-6-10 mM) levels, repectively. The experiment was conducted with 28 treatments each including 60 explants. Increasing concentration of NaCl stress, Ca ion concentration and the interaction of the two factors, were found to have significant effect (at 0.01 level) on number of regenerated shoots, fresh and dry weight of the cotyledonary tissue. The highest Ca ion concentration (10mM) improved the regeneration ability and the dry matter content of the tissue by decreasing salt stress effects.

Keywords