Phytochemical variations in sugar beet (Beta vulgaris L) in response to Ultraviolet-B radiation

Document Type : Scientific - Research


1 Ph.D student, Department of Biology, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Associate Professor, Department of Biology, University of Mohaghegh Ardabili, Ardabil, Iran.


All living organisms have mechanisms to deal with the harmful effects of ultraviolet (UV) radiation. In this study, the effects of three UV-B doses on some physiological properties of sugar beet cultivar, BR1 was evaluated in a completely randomized design with three replications. All plants were kept in a growth chamber at 25/20 ºC (day/night) with a 16 hours light and 8hours dark photoperiod for 30days. Then, plants were divided into 4 groups including control and three experimental groups exposed to 3.042, 6.084 and 9.126 KJm-2d-1UV-B radiation, respectively for one week. Results indicated that the highest amount of UV-B (9.126 KJm-2d-1) induced an 11% reduction in soluble sugar content of the leaf tissue. Treatment with 3.042, 6.084 and 9.126 KJm-2d-1UV-B resulted in 80, 82, and 86% increase in flavonoids content, respectively. Moreover, the highest amount of UV-B caused a 24% increase in total phenolic compounds content. Furthermore, UV-B radiation caused a significant increase in amounts of betalains (betanin and betaxanthin). The antioxidant activity of leaf methanolic extract increased under UV-B radiation. Evaluation of betaninand betaxanthin antioxidant activity also revealed that these pigments cause α, α-diphenyl-β-picrylhydrazyl free radical inhibition and betaxanthinhasa higher antioxidant activity than betanin. Increase in UV irradiation intensity increased the secondary metabolites and, consequently, increased the antioxidant capacity of the crop.


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