Pathogenic variability and genetic diversity of Fusarium solani isolates and its  association with sugarbeet root rot

Ashena, S.; Zamanizadeh, H. R.; Mahmoodi, S. B.

doi:10.22092/jsb.2008.1038

Pathogenic variability and genetic diversity of Fusarium solani isolates and its association with sugarbeet root rot

Document Type : Scientific - Research

Authors

¹ MSc. graduated in Islamic Azad University , Science and Research Branch , Iran

² Professor of Islamic Azad University , Science and Research Branch ,Tehran, Iran

³ Assistant Professor of Sugar Beet Seed Institute (SBSI) - Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

10.22092/jsb.2008.1038

Abstract

Diseased samples with root rot symptoms were collected from different sugar beet growing areas. From rotted roots, 20 fungal isolates were identified as Fusarium solani based on morphological characteristics .Pathogenecity of the isolates was studied in in vitro condition on sugar beet roots. The results showed pathogenic variability among the isolates. Cluster analysis divided the isolates into three pathogenic, moderate pathogenic and non-pathogenic groups. In pathogenicity test on 14 week old sugar beet plants no symptoms was observed in greenhouse conditions but the symptoms appeared on exposed roots .Genetic diversity of the isolates was studied using ITS4 and ITS5 primers through PCR-RFLP. Banding patterns of the isolates divided them into three groups including isolates with a single band (550 or 600 bp.), double band (550 and 600 bp.) and triple band (500, 550, and 600). PCR products of the isolates were digested with Msp1, EcoR1 and BamH1 restriction endonucleases. The results showed that BamH1 enzyme had no restriction site. DNA patterns of the isolates digested by EcoR1 were the same among all isolates and produced double monomorphic fragments for all isolates. Digestion with Msp1 showed intraspecific genetic diversity among different isolates of F.solani. The results didn’t show any relation among pathogenecity test, geographic location and ITS-rDNA analysis of the fungal isolates.

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